Bovine Neurotrophin 3/NTF3 ELISA Kit

96T/2480

HDNF; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; NGF 2; NGF2; NT 3; NT3; NTF3

Expression system for standard: NS0; Immunogen sequence: Y139-T257

There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.

<2pg/ml

15.6pg/ml-1000pg/ml

NTF3

Neurotrophin-3

P20783

Bovine Neurotrophin 3/NTF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0472-BV)

Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

20-25分钟

Catalog number	Description	Quantity
EK0472-BV-CAP	Anti-Bovine NTF3 Pre-coated 96-well strip microplate	1
EK0472-BV-ST	Bovine NTF3 Standard	10ng/tube×2
EK0472-BV-DA	Bovine NTF3 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	93	357	657	146	375	732
Standard deviation	4	17.5	34.2	7.88	22.9	47.58
CV (%)	4.3	4.9	5.2	5.4	6.1	6.5

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.073	0.148	0.277	0.561	0.870	1.686	2.529

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum