免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-R206
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse OSM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0479)
背景资料
OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0479-CAP | 96-well plate precoated with anti- mouse Oncostatin M antibody | 1 |
EK0479-ST | Lyophilized recombinant mouse Oncostatin M standard | 10ng/tube×2 |
EK0479-DA | Biotinylated anti- mouse Oncostatin M antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 61 | 260 | 822 | 59 | 280 | 850 |
Standard deviation | 4.51 | 16.64 | 36.16 | 5.31 | 22.4 | 52.7 |
CV(%) | 7.4% | 6.4% | 4.4% | 9% | 8% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.031 | 0.074 | 0.157 | 0.305 | 0.587 | 1.032 | 1.901 | 2.369 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)