Human TGF Alpha/TGFA ELISA Kit

96T/2480

EGF like TGF; ETGF; TFGA; TGF alpha; TGF type 1; TGFA

E.coli,V40-A89

There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%.

15.6 pg/ml - 1000 pg/ml

TGFA

transforming growth factor, alpha

P01135  

Human TGF Alpha/TGFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0511)

Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

25-30分钟

Catalog number	Description	Quantity
EK0511-CAP	96-well plate precoated with anti- human TGF alpha antibody	1
EK0511-ST	Lyophilized recombinant human TGF alpha standard	10ng/tubex2
EK0511-DA	Biotinylated anti- human TGF alpha antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	31	230	448	29	237	431
Standard deviation	1.98	16.56	34.49	2.4	18.96	34.04
CV(%)	6.4	7.2	7.7	8.3	8	7.9

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.102	0.199	0.291	0.443	0.711	1.156	1.676	2.315

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Human Milk

3.5h