产品名称
Mouse TNF Alpha/TNFA ELISA Kit
指标别名
Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor
免疫原
Expression system for standard: E.coli; Immunogen sequence: L80-L235
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor
文献引用格式
Mouse TNF Alpha/TNFA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0527)
背景资料
Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0527-CAP | 96-well plate precoated with anti- mouse TNF alpha antibody | 1 |
EK0527-ST | Lyophilized recombinant mouse TNF alpha standard | 10ng/tubex2 |
EK0527-DA | Biotinylated anti- mouse TNF alpha antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 50 | 299 | 16 | 53 | 305 |
Standard deviation | 0.82 | 3.9 | 22.12 | 0.91 | 4.39 | 24.4 |
CV(%) | 4.6% | 7.8% | 7.4% | 5.7% | 8.3% | 8% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.007 | 0.072 | 0.139 | 0.260 | 0.552 | 0.920 | 1.748 | 2.358 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)