产品名称
Mouse TNFR1/TNFRSF1A ELISA Kit
指标别名
CD120a; FPF; p55; p55 R; p60; TBP1; TBPI; TNF R; TNF R I; TNF R1; TNF R55; TNF Receptor 1; TNF Receptor I; TNF RI; TNFAR; TNFR I; TNFR1; TNFR55; TNFR60; TNFRSF1A
免疫原
Expression system for standard: NS0; Immunogen sequence: L30-A212
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor receptor superfamily member 1A
文献引用格式
Mouse TNFR1/TNFRSF1A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0529)
背景资料
Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0529-CAP | 96-well plate precoated with anti- mouse TNFR1 antibody | 1 |
EK0529-ST | Lyophilized recombinant mouse TNFR1 standard | 10ng/tube×2 |
EK0529-DA | Biotinylated anti- mouse TNFR1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 224 | 495 | 37 | 204 | 459 |
Standard deviation | 1.5 | 13.88 | 27.22 | 1.7 | 16.32 | 27.08 |
CV(%) | 4.3% | 6.2% | 5.5% | 4.6% | 8% | 5.9% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.036 | 0.110 | 0.215 | 0.442 | 0.822 | 1.328 | 1.876 | 2.152 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum