Human Cardiotrophin-1/CTF1 ELISA Kit

96T/2480

cardiotrophin 1; CT-1; CT1; CTF1; Cardiotrophin-1

E.coli, S2-A201

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10000 pg/ml

CTF1

cardiotrophin 1

Q16619  

Human Cardiotrophin-1/CTF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0563)

Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

15-20分钟

Catalog number	Description	Quantity
EK0563-CAP	96-well plate precoated with anti- human Cardiotrophin-1 antibody	1
EK0563-ST	Lyophilized recombinant human Cardiotrophin-1 standard	10ng/tubex2
EK0563-DA	Biotinylated anti- human Cardiotrophin-1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	253	924	3655	274	930	3410
Standard deviation	12.65	69.3	288.74	16.16	70.68	300.08
CV(%)	5	7.5	7.9	5.9	7.6	8.8

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.031	0.059	0.095	0.139	0.277	0.534	1.288	2.278

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h