产品名称
Mouse CCL1 ELISA Kit
指标别名
C-C motif chemokine ligand 1; I-309; P500; SCYA1; SISe; TCA3; CCL1
免疫原
Expression system for standard: NS0; Immunogen sequence: K24-C92
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 1
文献引用格式
Mouse CCL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0566)
背景资料
CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0566-CAP | 96-well plate precoated with anti- mouse CCL1 antibody | 1 |
EK0566-ST | Lyophilized recombinant mouse CCL1 standard | 10ng/tubex2 |
EK0566-DA | Biotinylated anti- mouse CCL1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 17 | 81 | 291 | 16 | 78 | 277 |
Standard deviation | 0.9 | 5.5 | 18.91 | 1.02 | 6.78 | 20.22 |
CV(%) | 5.3% | 6.8% | 6.5% | 6.4% | 8.7% | 7.3% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.029 | 0.098 | 0.157 | 0.318 | 0.574 | 1.144 | 1.630 | 2.067 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)