产品名称
Mouse CD30/TNFRSF8 ELISA Kit
指标别名
CD30; CD30L receptor; D1S166E; Ki 1; Ki 1 antigen; TNFRSF8
免疫原
Expression system for standard: NS0; Immunogen sequence: F19-T281
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor receptor superfamily member 8
文献引用格式
Mouse CD30/TNFRSF8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0570)
背景资料
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0570-CAP | 96-well plate precoated with anti- mouse CD30 antibody | 1 |
EK0570-ST | Lyophilized recombinant mouse CD30 standard | 10ng/tubex2 |
EK0570-DA | Biotinylated anti- mouse CD30 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 40 | 255 | 414 | 42 | 257 | 418 |
Standard deviation | 2.6 | 16.83 | 17.8 | 3.19 | 20.56 | 25.49 |
CV(%) | 6.5% | 6.6% | 4.3% | 7.6% | 8% | 6.1% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.081 | 0.148 | 0.235 | 0.505 | 1.010 | 1.482 | 2.158 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum