Mouse CD154/CD40LG ELISA Kit

96T/2480

CD154; CD40 L; CD40 ligand; CD40L; CD40L/CD154; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T BAM; T cell antigen Gp39; TNF related activation protein; TNFSF5; TRAP

Expression system for standard: NS0; Immunogen sequence: E61-L260

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

CD40LG

CD40 ligand

P27548  

Mouse CD154/CD40LG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0574)

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Catalog number	Description	Quantity
EK0574-CAP	96-well plate precoated with anti- mouse CD40L antibody	1
EK0574-ST	Lyophilized recombinant mouse CD40L standard	4ng/tubex2
EK0574-DA	Biotinylated anti- mouse CD40L antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	202	698	1026	185	716	1058
Standard deviation	13.93	39.08	79	14.06	52.26	85.69
CV(%)	6.9%	5.6%	7.7%	7.6%	7.3%	8.1%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.104	0.172	0.213	0.241	0.411	0.923	1.135	1.714

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h