产品名称
Human Prokineticin 1/PROK1 ELISA Kit
指标别名
prokineticin 1; EGVEGF; PK1; PRK1; PROK1; EG-VEGF
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human Prokineticin 1/PROK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0575)
背景资料
EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF(A20-F105), with molecular weight of 10.5 KDa.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0575-CAP | 96-well plate precoated with anti- human EG-VEGF antibody | 1 |
| EK0575-ST | Lyophilized recombinant human EG-VEGF standard | 10ng/tubex2 |
| EK0575-DA | Biotinylated anti- human EG-VEGF antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 249 | 474 | 31 | 228 | 490 |
| Standard deviation | 1.45 | 18.17 | 30.81 | 1.61 | 18.92 | 39.2 |
| CV(%) | 4.7 | 7.3 | 6.5 | 5.2 | 8.3 | 8 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.137 | 0.228 | 0.414 | 0.74 | 1.402 | 2.049 | 2.447 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)