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Human Prokineticin 1/PROK1 ELISA Kit

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EK0575

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human Prokineticin 1/PROK1 ELISA Kit
规格/价格
96T/2480
指标别名
prokineticin 1; EGVEGF; PK1; PRK1; PROK1; EG-VEGF
免疫原
NSO, A20-F105
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
PROK1
基因名全称
prokineticin 1
Uniprot ID
P58294  
文献引用格式
Human Prokineticin 1/PROK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0575)
背景资料
EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF(A20-F105), with molecular weight of 10.5 KDa.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0575-CAP96-well plate precoated with anti- human EG-VEGF antibody1
EK0575-STLyophilized recombinant human EG-VEGF standard10ng/tubex2
EK0575-DABiotinylated anti- human EG-VEGF antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3124947431228490
Standard deviation1.4518.1730.811.6118.9239.2
CV(%)4.77.36.55.28.38
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0750.1190.1650.2890.4160.8571.6182.454
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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