产品名称
Human RETN ELISA Kit
指标别名
ADSF; FIZZ3; HXCP1; resistin; RETN; RETN1; RSTN; XCP1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
文献引用格式
Human RETN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0581)
背景资料
Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0581-CAP | 96-well plate precoated with anti- human Resistin antibody | 1 |
EK0581-ST | Lyophilized recombinant human Resistin standard | 10ng/tubex2 |
EK0581-DA | Biotinylated anti- human Resistin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 135 | 639 | 2067 | 142 | 636 | 2227 |
Standard deviation | 8.1 | 40.25 | 161.22 | 8.52 | 46.42 | 175.93 |
CV(%) | 6 | 6.3 | 7.8 | 6 | 7.3 | 7.9 |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.015 | 0.122 | 0.202 | 0.450 | 0.743 | 1.242 | 1.650 | 2.072 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)