Rat TIMP1 ELISA Kit

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EK0583

96T ￥2280
货期：现货

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Rat TIMP-1 ELISA Kit standard curve
all(2)
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产品简介>

产品名称

Rat TIMP1 ELISA Kit

规格/价格

96T/2280

指标别名

CLGI; Collagenase inhibitor; EPA; EPO; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP 1; TIMP1; TIMP-1

免疫原

Expression system for standard: NS0; Immunogen sequence: C24-A217

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2pg/ml-2000pg/ml

基因名称

TIMP1

基因名全称

TIMP metallopeptidase inhibitor 1

Uniprot ID

P30120

文献引用格式

Rat TIMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0583)

背景资料

The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0583-CAP	96-well plate precoated with anti- rat TIMP-1 antibody	1
EK0583-ST	Lyophilized recombinant rat TIMP-1 standard	10ng/tubex2
EK0583-DA	Biotinylated anti- rat TIMP-1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	59	252	889	61	252	895
Standard deviation	4.24	13.1	45.33	5	13.35	46.54
CV(%)	7.2%	5.2%	5.1%	8.2%	5.3%	5.2%

典型数据示例

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.045	0.068	0.094	0.165	0.333	0.706	1.437	2.283

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat TIMP1 ELISA Kit

EK0583

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

其他介绍>

引用文献(0)>

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Rat TIMP1 ELISA Kit

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