产品名称
Human VEGFC ELISA Kit
指标别名
Flt4 L; Flt4 ligand; VEGF C; VEGFC; VRP
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
vascular endothelial growth factor C
文献引用格式
Human VEGFC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0588)
背景资料
Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0588-CAP | 96-well plate precoated with anti- human VEGF-C antibody | 1 |
EK0588-ST | Lyophilized recombinant human VEGF-C standard | 10ng/tubex2 |
EK0588-DA | Biotinylated anti- human VEGF-C antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 86 | 845 | 1351 | 89 | 836 | 1341 |
Standard deviation | 4.04 | 56.61 | 72.95 | 5.51 | 71.89 | 76.43 |
CV(%) | 4.7 | 6.7 | 5.4 | 6.2 | 8.6 | 5.7 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.016 | 0.067 | 0.114 | 0.213 | 0.363 | 0.794 | 1.524 | 2.332 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)