Human Cystatin C/CST3 ELISA Kit

96T|2480

ARMD11; CST3; Cystatin 3; cystatin C; CYTC; Gamma trace; Post gamma globulin

NSO, M1-A146

There is no detectable cross-reactivity with other relevant proteins.

312 pg/ml - 20000 pg/ml

CST3

cystatin C

P01034  

Human Cystatin C/CST3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0678)

Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

15-20分钟

Catalog number	Description	Quantity
EK0678-CAP	96-well plate precoated with anti- human Cystatin C antibody	1
EK0678-ST	Lyophilized recombinant human Cystatin C standard	20ng/tubex2
EK0678-DA	Biotinylated anti- human Cystatin C antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	687	2837	10374	647	2715	10112
Standard deviation	43.96	207.1	684.68	51.11	217.2	859.52
CV(%)	6.4	7.3	6.6	7.9	8	8.5

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.062	0.143	0.212	0.347	0.582	0.992	1.444	1.836

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk

3.5h