产品名称
Mouse CTACK/CCL27 ELISA Kit
指标别名
ALP; C C motif chemokine 27; CC chemokine ILC; CCL27; CTACK; CTAK; ESKINE; IL 11 R alpha locus chemokine; ILC; PESKY; SCYA27; Skinkine; Small inducible cytokine A27
免疫原
Expression system for standard: E.coli; Immunogen sequence: L26-N120
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 27
文献引用格式
Mouse CTACK/CCL27 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0689)
背景资料
Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). The human gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. And CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0689-CAP | 96-well plate precoated with anti- mouse CCL27 antibody | 1 |
EK0689-ST | Lyophilized recombinant mouse CCL27 standard | 10ng/tube×2 |
EK0689-DA | Biotinylated anti- mouse CCL27 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 180 | 414 | 1713 | 188 | 393 | 1657 |
Standard deviation | 9.18 | 24.01 | 99.35 | 11.84 | 29.47 | 107.7 |
CV(%) | 5.1% | 5.8% | 5.8% | 6.3% | 7.5% | 6.5% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.027 | 0.100 | 0.139 | 0.226 | 0.420 | 0.775 | 1.300 | 1.906 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)