Human B7-1/CD80 ELISA Kit

说明书

EK0707

96T ￥2480
货期：现货

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Human B7-1/CD80 ELISA Kit standard curve
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产品名称

Human B7-1/CD80 ELISA Kit

规格/价格

96T/2480

指标别名

Activation B7 1 antigen; B7; B7 1; B7-1; BB1; CD28LG; CD28LG1; CD80; CD80 molecule; CTLA 4 counter receptor B7.1; LAB7

免疫原

NSO, V35-N242

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

62.5 pg/ml - 4000 pg/ml

基因名称

CD80

基因名全称

CD80 molecule

Uniprot ID

P33681

文献引用格式

Human B7-1/CD80 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0707)

背景资料

The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0707-CAP	96-well plate precoated with anti- human CD80 antibody	1
EK0707-ST	Lyophilized recombinant human CD80 standard	10ng/tube×2
EK0707-DA	Biotinylated anti- human CD80 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	162	445	1656	163	425	1791
Standard deviation	7.29	34.71	69.55	7.98	34	100.29
CV(%)	4.5	7.8	4.2	4.9	8	5.6

典型数据示例

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.006	0.060	0.129	0.256	0.442	0.829	1.384	1.979

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human B7-1/CD80 ELISA Kit

EK0707

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Human B7-1/CD80 ELISA Kit

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