产品名称
Mouse B7-2/CD86 ELISA Kit
指标别名
Activation B7 2 antigen; B7 2; B70; BU63; CD28LG2; CD86; CD86 molecule; FUN 1; LAB72
免疫原
Expression system for standard: NS0; Immunogen sequence: V26-G245
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
T-lymphocyte activation antigen CD86
文献引用格式
Mouse B7-2/CD86 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0711)
背景资料
Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0711-CAP | 96-well plate precoated with anti- mouse CD86 antibody | 1 |
EK0711-ST | Lyophilized recombinant mouse CD86 standard | 10ng/tube×2 |
EK0711-DA | Biotinylated anti- mouse CD86 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 179 | 772 | 1639 | 165 | 834 | 1677 |
Standard deviation | 11.09 | 47.86 | 86.86 | 12.54 | 62.55 | 95.58 |
CV(%) | 6.2% | 6.2% | 5.3% | 7.6% | 7.5% | 5.7% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.067 | 0.125 | 0.170 | 0.307 | 0.525 | 0.897 | 1.449 | 2.211 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum