指标别名
C X C motif chemokine 1; CXCL1; FSP; GRO; GRO alpha(1 73); GRO1; GROa; Growth regulated alpha protein; MGSA; MGSA a; NAP 3; SCYB1
免疫原
Expression system for standard: E.coli; Immunogen sequence: A25-K96
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-X-C motif) ligand 1
文献引用格式
Rat CXCL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0724)
背景资料
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
试剂盒内容
Catalog number | Description | Quantity |
EK0724-CAP | 96-well plate precoated with anti- rat CXCL1 antibody | 1 |
EK0724-ST | Lyophilized recombinant rat CXCL1 standard | 10ng/tube×2 |
EK0724-DA | Biotinylated anti- rat CXCL1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 226 | 428 | 36 | 217 | 466 |
Standard deviation | 1.58 | 9.26 | 26.1 | 1.58 | 9.33 | 33.55 |
CV(%) | 4.4% | 4.1% | 6.1% | 4.4% | 4.3% | 7.2% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.044 | 0.130 | 0.206 | 0.376 | 0.646 | 1.088 | 1.646 | 2.101 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA)