产品名称
Rat MIP-2/CXCL2 ELISA Kit
指标别名
CINC 2a; C-X-C motif chemokine 2; Cxcl2; Gro2; GROb; GRO-beta-T; Mgsa b; MIP 2; MIP 2a; Mip2; MIP2-alpha; Scyb; Scyb2
免疫原
Expression system for standard: E.coli; Immunogen sequence: S32-N100
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-X-C motif) ligand 2
文献引用格式
Rat MIP-2/CXCL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0725)
背景资料
MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.
试剂盒内容
Catalog number | Description | Quantity |
EK0725-CAP | 96-well plate precoated with anti- rat MIP-2 antibody | 1 |
EK0725-ST | Lyophilized recombinant rat MIP-2 standard | 10ng/tube×2 |
EK0725-DA | Biotinylated anti- rat MIP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 171 | 537 | 31 | 178 | 563 |
Standard deviation | 2.69 | 7.86 | 21.48 | 2.88 | 8.36 | 27.02 |
CV(%) | 7.7% | 4.6% | 4% | 9.3% | 4.7% | 4.8% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.059 | 0.124 | 0.156 | 0.244 | 0.451 | 0.684 | 1.346 | 1.916 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)