产品名称
Human Endocan/ESM1 ELISA Kit
指标别名
Endothelial cell-specific molecule 1; ESM-1; ESM1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
endothelial cell-specific molecule 1
文献引用格式
Human Endocan/ESM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0752)
背景资料
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0752-CAP | 96-well plate precoated with anti- human ESM1 antibody | 1 |
EK0752-ST | Lyophilized recombinant human ESM1 standard | 10ng/tube×2 |
EK0752-DA | Biotinylated anti- human ESM1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 90 | 269 | 832 | 96 | 293 | 860 |
Standard deviation | 7.11 | 14.79 | 45.76 | 8.44 | 19.33 | 54.18 |
CV(%) | 7.9 | 5.5 | 5.5 | 8.8 | 6.6 | 6.3 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.076 | 0.136 | 0.262 | 0.502 | 0.968 | 1.695 | 2.361 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)