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Human Endocan/ESM1 ELISA Kit

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EK0752

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human Endocan/ESM1 ELISA Kit
规格/价格
96T/2480
指标别名
Endothelial cell-specific molecule 1; ESM-1; ESM1
免疫原
NSO, W20-R184
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
ESM1
基因名全称
endothelial cell-specific molecule 1
Uniprot ID
Q9NQ30  
文献引用格式
Human Endocan/ESM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0752)
背景资料
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0752-CAP96-well plate precoated with anti- human ESM1 antibody1
EK0752-STLyophilized recombinant human ESM1 standard10ng/tube×2
EK0752-DABiotinylated anti- human ESM1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)9026983296293860
Standard deviation7.1114.7945.768.4419.3354.18
CV(%)7.95.55.58.86.66.3
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0110.0760.1360.2620.5020.9681.6952.361
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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