Human Fetuin A/AHSG ELISA Kit

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EK0757

96T ￥2480
货期：现货

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Human Fetuin A ELISA Kit standard curve
all(2)
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产品名称

Human Fetuin A/AHSG ELISA Kit

规格/价格

96T/2480

指标别名

A2HS; AHS; AHSG; alpha 2 HS glycoprotein; Alpha 2 Z globulin; Ba alpha 2 glycoprotein; FETUA; Fetuin A; Fetuin-A; HSGA

免疫原

NSO, A19-V367

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

0.78 ng/ml - 50 ng/ml

基因名称

AHSG

基因名全称

alpha-2-HS-glycoprotein

Uniprot ID

P02765

文献引用格式

Human Fetuin A/AHSG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0757)

背景资料

Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0757-CAP	96-well plate precoated with anti- human Fetuin A antibody	1
EK0757-ST	Lyophilized recombinant human Fetuin A standard	50ng/tube×2
EK0757-DA	Biotinylated anti- human Fetuin A antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1646	4628	22095	1708	5021	21195
Standard deviation	126.74	185.12	883.8	143.47	225.94	1229.31
CV(%)	7.7	4	4	8.4	4.5	5.8

典型数据示例

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.062	0.189	0.273	0.430	0.731	1.274	1.944	2.408

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Fetuin A/AHSG ELISA Kit

EK0757

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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