产品名称
Human G-CSFR/CSF3R ELISA Kit
指标别名
CD114; CSF3R; G CSF R; G CSF receptor; GCSFR; G-CSFR; G-CSF-R
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
colony stimulating factor 3 receptor (granulocyte)
文献引用格式
Human G-CSFR/CSF3R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0766)
背景资料
The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0766-CAP | 96-well plate precoated with anti- human CSF3R antibody | 1 |
EK0766-ST | Lyophilized recombinant human CSF3R standard | 10ng/tube×2 |
EK0766-DA | Biotinylated anti- human CSF3R antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 72 | 222 | 823 | 69 | 240 | 838 |
Standard deviation | 5.54 | 9.99 | 59.25 | 6.27 | 15.12 | 67.87 |
CV(%) | 7.7 | 4.5 | 7.2 | 9.1 | 6.3 | 8.1 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.041 | 0.133 | 0.177 | 0.356 | 0.570 | 1.023 | 1.898 | 2.402 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)