产品名称
Human HAI-2/SPINT2 ELISA Kit
指标别名
DIAR3; FLJ45571; HAI-2; HAI2; Kop; PB
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
serine peptidase inhibitor, Kunitz type, 2
文献引用格式
Human HAI-2/SPINT2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0773)
背景资料
Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0773-CAP | 96-well plate precoated with anti- human HAI-2 antibody | 1 |
| EK0773-ST | Lyophilized recombinant human HAI-2 standard | 10ng/tube×2 |
| EK0773-DA | Biotinylated anti- human HAI-2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 54 | 205 | 935 | 56 | 220 | 990 |
| Standard deviation | 2.91 | 11.07 | 61.71 | 3.3 | 12.32 | 66.33 |
| CV(%) | 5.4 | 5.4 | 6.6 | 5.9 | 5.6 | 6.7 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.072 | 0.159 | 0.213 | 0.313 | 0.512 | 0.855 | 1.319 | 2.18 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Cell Lysates