产品名称
Mouse IL17D ELISA Kit
指标别名
IL 17D; IL 27; IL17D; IL-17D; IL27; interleukin 17D; Interleukin 27
免疫原
Expression system for standard: E.coli; Immunogen sequence: A25-R205
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL17D ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0792)
背景资料
Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0792-CAP | 96-well plate precoated with anti- mouse IL-17D antibody | 1 |
EK0792-ST | Lyophilized recombinant mouse IL-17D standard | 10ng/tube×2 |
EK0792-DA | Biotinylated anti- mouse IL-17D antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 147 | 775 | 1330 | 157 | 700 | 1222 |
Standard deviation | 5.88 | 51.15 | 98.42 | 8.79 | 50.4 | 97.76 |
CV(%) | 4% | 6.6% | 7.4% | 5.6% | 7.2% | 8% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.042 | 0.106 | 0.158 | 0.253 | 0.460 | 0.883 | 1.530 | 2.288 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)