产品名称
Mouse IL21 ELISA Kit
指标别名
IL 21; IL21; IL-21; interleukin 21; Za11
免疫原
Expression system for standard: E.coli; Immunogen sequence: P25-S146
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL21 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0797)
背景资料
Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T (H)17 cells.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0797-CAP | 96-well plate precoated with anti- mouse IL-21antibody | 1 |
EK0797-ST | Lyophilized recombinant mouse IL-21 standard | 10ng/tube×2 |
EK0797-DA | Biotinylated anti- mouse IL-21 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 176 | 862 | 1322 | 188 | 946 | 1262 |
Standard deviation | 9.32 | 43.96 | 96.5 | 9.96 | 52.97 | 107.27 |
CV(%) | 5.3% | 5.1% | 7.3% | 5.3% | 5.6% | 8.5% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.061 | 0.093 | 0.131 | 0.239 | 0.479 | 0.837 | 1.677 | 2.361 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum