产品名称
Human M-CSFR/CSF1R ELISA Kit
指标别名
C FMS; CD115; CSF 1 R; CSF1R; CSFR; FIM2; FMS; Proto oncogene c Fms
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
colony stimulating factor 1 receptor
文献引用格式
Human M-CSFR/CSF1R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0807)
背景资料
CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5(5q32) on the Crick(minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0807-CAP | 96-well plate precoated with anti- human CSF1R antibody | 1 |
EK0807-ST | Lyophilized recombinant human CSF1R standard | 10ng/tube×2 |
EK0807-DA | Biotinylated anti- human CSF1R antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 131 | 701 | 1183 | 140 | 669 | 1090 |
Standard deviation | 5.76 | 55.37 | 67.43 | 6.58 | 60.21 | 70.85 |
CV(%) | 4.4 | 7.9 | 5.7 | 4.7 | 9 | 6.5 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.060 | 0.150 | 0.219 | 0.338 | 0.565 | 0.883 | 1.502 | 2.121 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)