产品名称
Human KGF/FGF7 ELISA Kit
指标别名
Fibroblast growth factor 7; HBGF-7; KGF; FGF7
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
fibroblast growth factor 7
文献引用格式
Human KGF/FGF7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0820)
背景资料
Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0820-CAP | 96-well plate precoated with anti- human FGF7 antibody | 1 |
EK0820-ST | Lyophilized recombinant human FGF7 standard | 10ng/tube×2 |
EK0820-DA | Biotinylated anti- human FGF7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 100 | 261 | 837 | 97 | 261 | 764 |
Standard deviation | 7.5 | 16.18 | 41.85 | 7.66 | 20.35 | 45.84 |
CV(%) | 7.5 | 6.2 | 5 | 7.9 | 7.8 | 6 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.070 | 0.104 | 0.129 | 0.191 | 0.337 | 0.635 | 1.348 | 2.100 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)