产品名称
Human RAGE/AGER ELISA Kit
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78 pg/ml - 5000 pg/ml
基因名全称
advanced glycosylation end product-specific receptor
文献引用格式
Human RAGE/AGER ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0827)
背景资料
RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0827-CAP | 96-well plate precoated with anti- human Rage antibody | 1 |
EK0827-ST | Lyophilized recombinant human Rage standard | 10ng/tube×2 |
EK0827-DA | Biotinylated anti- human Rage antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 154 | 623 | 3076 | 159 | 595 | 3344 |
Standard deviation | 10.16 | 27.41 | 221.47 | 10.65 | 32.72 | 250.8 |
CV(%) | 6.6 | 4.4 | 7.2 | 6.7 | 5.5 | 7.5 |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.075 | 0.166 | 0.291 | 0.391 | 0.875 | 1.375 | 1.867 | 2.153 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)