产品名称
Human tPA/PLAT ELISA Kit
指标别名
Alteplase; plasminogen activator; tissue; PLAT; Reteplase; T PA; t plasminogen activator; Tissue Plasminogen Activator; tPA
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
plasminogen activator, tissue
文献引用格式
Human tPA/PLAT ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0897)
背景资料
Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0897-CAP | 96-well plate precoated with anti- human PLAT antibody | 1 |
EK0897-ST | Lyophilized recombinant human PLAT standard | 10ng/tube×2 |
EK0897-DA | Biotinylated anti- human PLAT antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 84 | 237 | 975 | 81 | 229 | 878 |
Standard deviation | 5.37 | 15.4 | 43.87 | 5.58 | 17.63 | 43.02 |
CV(%) | 6.4 | 6.5 | 4.5 | 6.9 | 7.7 | 4.9 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.029 | 0.106 | 0.193 | 0.337 | 0.614 | 1.066 | 1.751 | 2.326 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine