Catalog number | Description | Quantity |
---|---|---|
EK0904-CAP | 96-well plate precoated with anti- human CEA antibody | 1 |
EK0904-ST | Lyophilized recombinant human CEA standard | 20ng/tube×2 |
EK0904-DA | Biotinylated anti- human CEA antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 776 | 1668 | 10176 | 724 | 1662 | 10501 |
Standard deviation | 39.57 | 125.1 | 407.04 | 39.09 | 137.94 | 420.04 |
CV(%) | 5.1 | 7.5 | 4 | 5.4 | 8.3 | 4 |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.025 | 0.155 | 0.278 | 0.531 | 0.961 | 1.531 | 2.114 | 2.531 |
Human CEA ELISA Kit standard curve
Human CEA ELISA Kit standard curve