Catalog number | Description | Quantity |
---|---|---|
EK0905-CAP | 96-well plate precoated with anti- human Angiostatin K1-3 antibody | 1 |
EK0905-ST0 | Lyophilized recombinant human Angiostatin K1-3 standard | 100ng/tube×2 |
EK0905-DA | Biotinylated anti- human Angiostatin K1-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 241 | 1987 | 5716 | 218 | 1908 | 5845 |
Standard deviation | 12.05 | 79.48 | 337.24 | 13.73 | 91.58 | 432.53 |
CV(%) | 5 | 4 | 5.9 | 6.3 | 4.8 | 7.4 |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.015 | 0.132 | 0.253 | 0.423 | 0.746 | 1.355 | 1.827 | 2.310 |
Human Angiostatin K1-3 ELISA Kit standard curve
Human Angiostatin K1-3 ELISA Kit standard curve