产品名称
Human Angiostatin K1-3/PLG ELISA Kit
指标别名
Angiostatin; plasminogen; PLG
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Angiostatin K1-3/PLG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0905)
背景资料
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED =70 nM) relative to kringles 1-4(ED = 135 nM).
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0905-CAP | 96-well plate precoated with anti- human Angiostatin K1-3 antibody | 1 |
| EK0905-ST0 | Lyophilized recombinant human Angiostatin K1-3 standard | 10ng/tube×2 |
| EK0905-DA | Biotinylated anti- human Angiostatin K1-3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 241 | 1987 | 5716 | 218 | 1908 | 5845 |
| Standard deviation | 12.05 | 79.48 | 337.24 | 13.73 | 91.58 | 432.53 |
| CV(%) | 5 | 4 | 5.9 | 6.3 | 4.8 | 7.4 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.044 | 0.11 | 0.182 | 0.328 | 0.575 | 0.969 | 1.498 | 2.042 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)