产品名称
Human TLR3 ELISA Kit
指标别名
CD283; TLR3; toll like receptor 3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
toll-like receptor 3
文献引用格式
Human TLR3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0907)
背景资料
Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0907-CAP | 96-well plate precoated with anti- human TLR3 antibody | 1 |
| EK0907-ST | Lyophilized recombinant human TLR3 standard | 10ng/tube×2 |
| EK0907-DA | Biotinylated anti- human TLR3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 290 | 1771 | 5327 | 301 | 1733 | 5141 |
| Standard deviation | 14.21 | 125.74 | 292.98 | 18.96 | 152.5 | 375.29 |
| CV(%) | 4.9 | 7.1 | 5.5 | 6.3 | 8.8 | 7.3 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.06 | 0.134 | 0.206 | 0.373 | 0.66 | 1.049 | 1.574 | 2.038 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)