产品名称
Mouse CD23/FCER2 ELISA Kit
指标别名
BLAST 2; CD23; CD23A; CLEC4J; Fc epsilon RII; FCE2; FCER2; IGEBF; Lymphocyte IgE receptor
免疫原
Expression system for standard: NS0; Immunogen sequence: E50-P331
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Low affinity immunoglobulin epsilon Fc receptor
文献引用格式
Mouse CD23/FCER2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0924)
背景资料
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0924-CAP | 96-well plate precoated with anti- mouse CD23 antibody | 1 |
| EK0924-ST | Lyophilized recombinant mouse CD23 standard | 10ng/tube×2 |
| EK0924-DA | Biotinylated anti- mouse CD23 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 256 | 1831 | 5853 | 234 | 1881 | 5493 |
| Standard deviation | 11 | 137.32 | 345.32 | 12.87 | 156.12 | 373.52 |
| CV(%) | 4.3% | 7.5% | 5.9% | 5.5% | 8.3% | 6.8% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.068 | 0.121 | 0.179 | 0.28 | 0.484 | 0.867 | 1.411 | 1.892 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)