产品名称
Human Tissue Factor/F3 ELISA Kit
指标别名
CD142; Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
coagulation factor III (thromboplastin, tissue factor)
文献引用格式
Human Tissue Factor/F3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0928)
背景资料
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0928-CAP | 96-well plate precoated with anti- human Tissue factor antibody | 1 |
EK0928-ST | Lyophilized recombinant human Tissue factor standard | 10ng/tube×2 |
EK0928-DA | Biotinylated anti- human Tissue factor antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 28 | 204 | 455 | 28 | 205 | 493 |
Standard deviation | 1.26 | 13.46 | 34.58 | 1.54 | 14.14 | 40.91 |
CV(%) | 4.5 | 6.6 | 7.6 | 5.5 | 6.9 | 8.3 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.035 | 0.090 | 0.155 | 0.254 | 0.479 | 0.914 | 1.446 | 2.308 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate), Cell Lysates, and Urine