产品名称
Human IL22 ELISA Kit
指标别名
Cytokine Zcyto18; IL 21; IL 22; IL D110; IL TIF; IL21; IL22; IL-22; ILTIF; interleukin 22; TIFa; TIFIL 23; zcyto18
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human IL22 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0933)
背景资料
Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0933-CAP | 96-well plate precoated with anti- human IL-22 antibody | 1 |
| EK0933-ST | Lyophilized recombinant human IL-22 standard | 10ng/tube×2 |
| EK0933-DA | Biotinylated anti- human IL-22 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 40 | 215 | 435 | 41 | 220 | 427 |
| Standard deviation | 2.04 | 14.83 | 26.97 | 2.58 | 18.92 | 27.32 |
| CV(%) | 5.1 | 6.9 | 6.2 | 6.3 | 8.6 | 6.4 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.016 | 0.112 | 0.226 | 0.37 | 0.643 | 1.114 | 1.566 | 1.925 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)