产品名称
Human TSLP ELISA Kit
指标别名
thymic stromal lymphopoietin; TSLP
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
thymic stromal lymphopoietin
文献引用格式
Human TSLP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0958)
背景资料
Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0958-CAP | 96-well plate precoated with anti- human TSLP antibody | 1 |
EK0958-ST | Lyophilized recombinant human TSLP standard | 10ng/tube×2 |
EK0958-DA | Biotinylated anti- human TSLP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 89 | 239 | 883 | 94 | 243 | 964 |
Standard deviation | 4.36 | 17.92 | 45.91 | 5.82 | 19.92 | 65.55 |
CV(%) | 4.9 | 7.5 | 5.2 | 6.2 | 8.2 | 6.8 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.015 | 0.078 | 0.146 | 0.270 | 0.515 | 0.892 | 1.485 | 2.220 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)