产品名称
Human ANGPTL4 ELISA Kit
指标别名
angiopoietin like 4; Angiopoietin like protein 4; Angiopoietin related protein 4; ANGPTL4; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PSEC0166; UNQ171/PRO197
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
625 pg/ml - 40000 pg/ml
基因名全称
angiopoietin-like 4
文献引用格式
Human ANGPTL4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0960)
背景资料
Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0960-CAP | 96-well plate precoated with anti- human ANGPTL4 antibody | 1 |
EK0960-ST | Lyophilized recombinant human ANGPTL4 standard | 40ng/tube×2 |
EK0960-DA | Biotinylated anti- human ANGPTL4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1114 | 4583 | 20920 | 1120 | 4790 | 21489 |
Standard deviation | 73.52 | 256.64 | 941.4 | 79.52 | 320.93 | 988.49 |
CV(%) | 6.6 | 5.6 | 4.5 | 7.1 | 6.7 | 4.6 |
典型数据示例
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.001 | 0.201 | 0.350 | 0.599 | 0.929 | 1.351 | 1.725 | 2.196 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)