Bovine TGF beta 2/TGFB2 ELISA Kit

96T/2480

BSC 1 cell growth inhibitor; Cetermin; G TSF; Polyergin; TGF beta 2; TGF beta2; TGFB2; TGF-beta 2; TGFβ2; TGF-β2

Expression system for standard: NS0; Immunogen sequence: A303-S414

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

31.2pg/ml-2000pg/ml

TGFB2

Transforming growth factor beta-2

P61812

Bovine TGF beta 2/TGFB2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0981-BV)

Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured Bovine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

20-25分钟

Catalog number	Description	Quantity
EK0981-BV-CAP	Anti-Bovine TGFB2 Pre-coated 96-well strip microplate	1
EK0981-BV-ST	Bovine TGFB2 Standard	10ng/tube×2
EK0981-BV-DA	Bovine TGFB2 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	221	564	1145	236	576	1095
Standard deviation	13.7	31.6	46.9	15.8	33.41	70.1
CV (%)	6.2	5.6	4.1	6.7	5.8	6.4

Concentration(pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.031	0.094	0.157	0.345	0.487	1.032	1.901	2.369

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)