产品名称
Human TACI/TNFRSF13B ELISA Kit
指标别名
TNF receptor superfamily member 13B; CD267; CVID; CVID2; IGAD2; RYZN; TACI; TNFRSF14B; TNFRSF13B
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.7 pg/ml - 6000 pg/ml
基因名全称
tumor necrosis factor receptor superfamily, member 13B
文献引用格式
Human TACI/TNFRSF13B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0986)
背景资料
Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0986-CAP | 96-well plate precoated with anti- human TNFRSF13B antibody | 1 |
EK0986-ST | Lyophilized recombinant human TNFRSF13B standard | 10ng/tube×2 |
EK0986-DA | Biotinylated anti- human TNFRSF13B antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 180 | 907 | 2596 | 162 | 875 | 2578 |
Standard deviation | 13.68 | 39 | 134.99 | 13.12 | 48.12 | 167.57 |
CV(%) | 7.6 | 4.3 | 5.2 | 8.1 | 5.5 | 6.5 |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.036 | 0.112 | 0.160 | 0.302 | 0.514 | 0.977 | 1.686 | 2.343 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)