产品名称
Mouse MMP12 ELISA Kit
指标别名
HME; Macrophage elastase; Macrophage metalloelastase; Matrix metalloproteinase 12; ME; MME; MMP 12; MMP12
免疫原
Expression system for standard: NS0; Immunogen sequence: A18-C462
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Macrophage metalloelastase
文献引用格式
Mouse MMP12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0996)
背景资料
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0996-CAP | 96-well plate precoated with anti- mouse MMP-12 antibody | 1 |
EK0996-ST | Lyophilized recombinant mouse MMP-12 standard | 10ng/tube×2 |
EK0996-DA | Biotinylated anti- mouse MMP-12 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 127 | 628 | 1430 | 134 | 684 | 1370 |
Standard deviation | 9.27 | 46.47 | 100.1 | 11.12 | 57.45 | 97.27 |
CV(%) | 7.3% | 7.4% | 7% | 8.3% | 8.4% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.013 | 0.068 | 0.119 | 0.202 | 0.409 | 0.787 | 1.459 | 2.189 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)