Chicken TGF Beta 3/TGFB3 ELISA Kit

96T/2480

ARVD; TGF beta 3; TGF beta3; TGFB3; TGF-beta 3; TGF-β3

Expression system for standard: NS0; Immunogen sequence: A301-S412

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

31.2pg/ml-2000pg/ml

TGFB3

Transforming growth factor beta-3

P10600

Chicken TGF Beta 3/TGFB3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1103-CH)

Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.

25-30分钟

Catalog number	Description	Quantity
EK1103-CH-CAP	Anti-Chicken TGFB3 Pre-coated 96-well strip microplate	1
EK1103-CH-ST	Chicken TGFB3 Standard	10ng/tube×2
EK1103-CH-DA	Chicken TGFB3 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	271	631	1153	294	670	1265
Standard deviation	14.4	29	45	18.82	36.4	72.85
CV (%)	5.3	4.6	3.9	6.4	5.7	5.8

Concentration(pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.035	0.121	0.22	0.391	0.671	1.043	1.608	2.051

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

chicken

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (EDTA) and Urine