Human Alpha-2-Macroglobulin/A2M ELISA Kit

96T/2480

A2M; Alpha 2 M; alpha 2 macroglobulin; CPAMD5; FWP007; S863 7; α-2M

from human plasma

There is no detectable cross-reactivity with other relevant proteins.

625 pg/ml - 40000 pg/ml

A2M

alpha-2-macroglobulin

P01023  

Human Alpha-2-Macroglobulin/A2M ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1118)

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

15-20分钟

Catalog number	Description	Quantity
EK1118-CAP	96-well plate precoated with anti- human A2M antibody	1
EK1118-ST	Lyophilized recombinant human A2M standard	40ng/tube×2
EK1118-DA	Biotinylated anti- human A2M antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1367	3876	26784	1316	3942	26889
Standard deviation	97.05	236.43	2062.36	103.96	240.46	2151.12
CV(%)	7.1	6.1	7.7	7.9	6.1	8

Concentration(pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.075	0.129	0.203	0.332	0.575	1.108	1.477	2.135

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h