产品名称
Human Azurocidin/AZU1 ELISA Kit
指标别名
azurocidin 1; AZAMP; AZU; CAP37; HBP; HUMAZUR; NAZC; Hhbp; Azurocidin; AZU1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human Azurocidin/AZU1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1161)
背景资料
Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1161-CAP | 96-well plate precoated with anti- human Azurocidin antibody | 1 |
EK1161-ST | Lyophilized recombinant human Azurocidin standard | 10ng/tube×2 |
EK1161-DA | Biotinylated anti- human Azurocidin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 178 | 606 | 1031 | 175 | 567 | 1093 |
Standard deviation | 9.79 | 39.99 | 75.26 | 9.62 | 38.55 | 96.18 |
CV(%) | 5.5 | 6.6 | 7.3 | 5.5 | 6.8 | 8.8 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.015 | 0.046 | 0.070 | 0.121 | 0.293 | 0.650 | 1.514 | 2.260 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)