产品名称
Human S100A12 ELISA Kit
指标别名
CAAF1; CAGC; Calgranulin C; CGRP; EN RAGE; ENRAGE; MRP6; Neutrophil S100 protein; p6; Protein S100 A12; S100A12
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
S100 calcium binding protein A12
文献引用格式
Human S100A12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1169)
背景资料
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1169-CAP | 96-well plate precoated with anti- human S100A12 antibody | 1 |
EK1169-ST | Lyophilized recombinant human S100A12 standard | 10ng/tube×2 |
EK1169-DA | Biotinylated anti- human S100A12 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 58 | 265 | 947 | 56 | 247 | 855 |
Standard deviation | 2.32 | 20.4 | 67.23 | 3.08 | 23.46 | 68.4 |
CV(%) | 4 | 7.7 | 7.1 | 5.5 | 9.5 | 8 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.021 | 0.240 | 0.584 | 0.901 | 1.399 | 1.548 | 1.929 | 2.276 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)