产品名称
Mouse ST2/IL1RL1 ELISA Kit
指标别名
DER4; FIT 1; IL1RL1; IL-1RL1; IL-33R; interleukin 1 receptor like 1; Protein ST2; ST2; ST2L; ST2V; T1; IL 1RL1
免疫原
Expression system for standard: NS0; Immunogen sequence: S27-R332
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Interleukin-1 receptor-like 1
文献引用格式
Mouse ST2/IL1RL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1180)
背景资料
Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1180-CAP | 96-well plate precoated with anti- mouse IL1RL1 antibody | 1 |
EK1180-ST | Lyophilized recombinant mouse IL1RL1 standard | 10ng/tube×2 |
EK1180-DA | Biotinylated anti- mouse IL1RL1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 194 | 1525 | 5484 | 189 | 1538 | 5883 |
Standard deviation | 8.73 | 94.55 | 296.13 | 10.77 | 115.35 | 394.16 |
CV(%) | 4.5% | 6.2% | 5.4% | 5.7% | 7.5% | 6.7% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.066 | 0.098 | 0.164 | 0.294 | 0.544 | 1.007 | 1.821 | 2.388 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)