产品名称
Mouse Periostin/POSTN ELISA Kit
指标别名
OSF 2; OSF2; Osteoblast specific factor 2; PDLPOSTN; Periostin; PN; POSTN; RP11 412K4.1
免疫原
Expression system for standard: NS0; Immunogen sequence: N24-Q836
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Periostin/POSTN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1187)
背景资料
Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1187-CAP | 96-well plate precoated with anti- mouse Periostin antibody | 1 |
EK1187-ST | Lyophilized recombinant mouse Periostin standard | 10ng/tube×2 |
EK1187-DA | Biotinylated anti- mouse Periostin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 109 | 795 | 2510 | 119 | 740 | 2598 |
Standard deviation | 6.97 | 53.26 | 110.44 | 8.33 | 62.9 | 119.5 |
CV(%) | 6.4% | 6.7% | 4.4% | 7% | 8.5% | 4.6% |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.004 | 0.063 | 0.132 | 0.269 | 0.564 | 0.985 | 1.681 | 2.421 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)