产品名称
Mouse SAA/SAA1 ELISA Kit
指标别名
serum amyloid A1; PIG4; SAA; SAA2; TP53I4; SAA1
免疫原
Expression system for standard: E.coli; Immunogen sequence: G20-Y122
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
基因名全称
Serum amyloid A-1 protein
文献引用格式
Mouse SAA/SAA1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1190)
背景资料
Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1190-CAP | 96-well plate precoated with anti- mouse SAA antibody | 1 |
| EK1190-ST0 | Lyophilized recombinant mouse SAA standard | 100ng/tube×2 |
| EK1190-DA | Biotinylated anti- mouse SAA antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 270 | 1129 | 2924 | 194 | 1044 | 3393 |
| Standard deviation | 9.26 | 70.9 | 166.67 | 9.72 | 52.93 | 245.81 |
| CV(%) | 3.4% | 6.3% | 5.7% | 5% | 5.1% | 7.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.001 | 0.093 | 0.175 | 0.322 | 0.578 | 0.971 | 1.609 | 1.938 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine