Human Trefoil factor 1/TFF1 ELISA Kit

96T/2480

D21S21; HP1.A; HPS2; pNR 2; Polypeptide P1.A; Protein pS2; pS2; TFF1; trefoil factor 1

E.coli, E25-F84

There is no detectable cross-reactivity with other relevant proteins.

15.6 pg/ml - 1000 pg/ml

TFF1

trefoil factor 1

P04155  

Human Trefoil factor 1/TFF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1232)

TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

25-30分钟

Catalog number	Description	Quantity
EK1232-CAP	96-well plate precoated with anti- human TFF1 antibody	1
EK1232-ST	Lyophilized recombinant human TFF1 standard	1ng/tube×2
EK1232-DA	Biotinylated anti- human TFF1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	35	177	433	38	193	420
Standard deviation	1.96	9.91	29.87	2.16	11	29.4
CV(%)	5.6	5.6	6.9	5.7	5.7	7

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.015	0.042	0.066	0.110	0.231	0.457	1.017	2.396

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h