产品名称
Human Trefoil factor 3/TFF3 ELISA Kit
指标别名
HITF; hP1.B; Intestinal trefoil factor; ITF; Polypeptide P1.B; TFF3; TFI; Trefoil factor 3; trefoil factor 3 (intestinal)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
trefoil factor 3 (intestinal)
文献引用格式
Human Trefoil factor 3/TFF3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1234)
背景资料
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1234-CAP | 96-well plate precoated with anti- human TFF3 antibody | 1 |
EK1234-ST | Lyophilized recombinant human TFF3 standard | 10ng/tube×2 |
EK1234-DA | Biotinylated anti- human TFF3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 92 | 646 | 1668 | 89 | 679 | 1553 |
Standard deviation | 5.06 | 41.34 | 78.39 | 5.69 | 51.6 | 100.94 |
CV(%) | 5.5 | 6.4 | 4.7 | 6.4 | 7.6 | 6.5 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.100 | 0.166 | 0.234 | 0.317 | 0.542 | 0.901 | 1.485 | 2.286 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Urine