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Human TATI/SPINK1 ELISA Kit

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EK1241

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human TATI/SPINK1 ELISA Kit
规格/价格
96T/2480
指标别名
PCTT; PSTI; SPINK1; Spink3; TaTI; TCP
免疫原
E.coli, D24-C79
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
SPINK1
基因名全称
serine peptidase inhibitor, Kazal type 1
Uniprot ID
P00995  
文献引用格式
Human TATI/SPINK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1241)
背景资料
Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1241-CAP96-well plate precoated with anti- human SPINK1 antibody1
EK1241-STLyophilized recombinant human SPINK1 standard10ng/tube×2
EK1241-DABiotinylated anti- human SPINK1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2451862581725417846136
Standard deviation16.9135.92418.8217.78148.07466.33
CV(%)6.97.37.278.37.6
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0340.1200.1940.3410.6230.9891.6342.273
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)
实验时长
3.5h

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