产品名称
Human TATI/SPINK1 ELISA Kit
指标别名
PCTT; PSTI; SPINK1; Spink3; TaTI; TCP
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
serine peptidase inhibitor, Kazal type 1
文献引用格式
Human TATI/SPINK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1241)
背景资料
Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1241-CAP | 96-well plate precoated with anti- human SPINK1 antibody | 1 |
EK1241-ST | Lyophilized recombinant human SPINK1 standard | 10ng/tube×2 |
EK1241-DA | Biotinylated anti- human SPINK1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 245 | 1862 | 5817 | 254 | 1784 | 6136 |
Standard deviation | 16.9 | 135.92 | 418.82 | 17.78 | 148.07 | 466.33 |
CV(%) | 6.9 | 7.3 | 7.2 | 7 | 8.3 | 7.6 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.034 | 0.120 | 0.194 | 0.341 | 0.623 | 0.989 | 1.634 | 2.273 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)